In development 3D Cell Culture Vessel: MicoCell™

Features and Properties

Examples of Use

Vessels Examples

Culture Examples

Human adipose-derived stem cells

Rat Primary Hepatocytes

Liver Cancer Cells (HepG2)

Cell Aggregates Containing Vascular Structures

Co-culture of adipose-derived stem cells and vascular endothelial cells (HUVEC)
• Forming a dome-shaped blood vessel in the vertical direction from the adhesive substrate surface

• Vascular structure improves hypoxia (state of low oxygen) in cell aggregates

Item	Features	Advantages
Vessel Properties	No use of biologically-derived components and materials	Possible to create cultures that do not contain biologically-derived components and materials
	Flat bottom, high transparency, no surface treatment	Clear observation with phase contrast and fluorescence microscopes
	High-density cavity structure	Possible to produce large quantities of cell aggregates with a uniform particle size (several hundred to 40,000 or more)
Culture Properties	Formation of cell aggregates that are moderately attached to the bottom of the vessel	Reduces loss of cell aggregates during culture medium exchange, enables long-term culturing, and is easy to carry
	Cell aggregates can be collected without the use of release agents (enzymatic processing)	Easy operation, low toxicity, cell products such as extracellular matrix
Cells Functions	Maintains a good survival rate and protein production	Applications in drug screening, regenerative medicine, etc.

Cautions

• This is a product we are currently developing.
  It is not a medical device, but a physical and chemical device for laboratory research.
• The data listed in this page are examples, and are not guarantees of the performance of this product.
• Please note that the contents and MicoCell™ specifications listed on this page may be changed without prior notice.
• Please be aware of the intellectual property rights related to third-party usage, usage methods, etc. when using the Nippon Shokubai product(s) listed in this website page. The Company does not take any responsibility whatsoever for any issues that may arise in relation to these intellectual property rights.

Related Papers

Fumiaki Shima, Tomomi Makino, Fabrication of Spheroids with Dome-Shaped Endothelial Tube Networks by an Adhesive Culture System, Adv. Biosyst. 2000120 (2020) https://doi.org/10.1002/adbi.202000120

Yutaro Yamada et al., 3D-cultured small size adipose-derived stem cell spheroids promote bone regeneration in the critical-sized bone defect rat model, Biochem. Biophys. Res. Commun. 603, 57-62 (2022) https://doi.org/10.1016/j.bbrc.2022.03.027

Fumiaki Shima, Tomomi Makino, Construction of brain-like spheroids containing endothelial tubular networks by an adhesive culture system, Biochem. Biophys. Res. Commun. 623, 176-180(2022) https://doi.org/10.1016/j.bbrc.2022.07.063

Minoru Kishikawa et al.,Bone regeneration using three-dimensional spheroids derived from dedifferentiated fat cells, Oral Sci Int. 2025;22(1):e128 https://doi.org/10.1002/osi2.1286

Sae Yokomine et al., Floating or adherent hepatocyte spheroid cultures using microwell chips with polyethylene glycol or polyimide surfaces, Biomed. Mater. 20 (2025) 035009 https://doi.org/10.1088/1748-605X/adc17d

Akihiko Yamaguchi et al., Coconut oil works as a gonadal feedback testosterone booster and increases pituitary aromatase (cyp19a1b) expression levels in fugu (Takifugu rubripes), Aquaculture 613(2026)743310 https://doi.org/10.1016/j.aquaculture.2025.743310

Satoshi Sobajima et al., Preclinical and early clinical safety of intra-articular spheroid adipose-derived stem cells for knee osteoarthritis: A translational study, Osteoarthritis and Cartilage Open 8 (2026) 100792 https://doi.org/10.1016/j.ocarto.2026.100792

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